3 DAYS MASTERCLASS ON
Insilico Metagenomics & Metatranscriptomics Analysis
A Comprehensive & Intensive Hands-On Command line software based Offline Training program in New Delhi
Intensive Learning
Hands-On Exercise
Offline Sessions
Individual Tasks
"OFFLINE HAND-ON INTENSIVE PROGRAM"
Introduction to Masterclass
Metagenomics is the study of genetic material recovered directly from environmental samples. This is useful when attempting to understand what microbes are present and what they are doing in a particular environment.
Advancements in Next Generation Sequencing (NGS) technologies regarding throughput, read length and accuracy had a major impact on microbiome research by significantly improving 16S rRNA amplicon sequencing.
Metatranscriptomic Sequencing provides direct access to culturable and non-culturable microbial transcriptome information by large-scale, high-throughput sequencing of transcripts from all microbial communities in specific environmental samples.
Metatranscriptomic Sequencing offers an opportunity to randomly sequence mRNAs as a unit for understanding the regulation of complex processes in microbial communities.
Applications of Metagenomics & Metatranscriptomics
In this section we are discussing some of the potential areas of application of Metagenomics & Metatranscriptomics analysis & how it can be helpful in these areas to carryout analysis.
- Agriculture; for analyzing the soil quality
- Energy; Biofuel, Fuelcell Etc.
- Food & Nutrition; Dairy, Wine Etc.
- BioSensor; Enzyme Estimation & assay Etc.
- Environment; Biodegradation, Etc.
Different Types of Analysis
Majorly, there are 3 different types of Metagenomics & Metatranscriptomics Analysis so below we have tried to list them down to help you take better decision if this masterclass will be relevant for your learning and be helpful in your intended area of research.
Targeted Metagenomics
certain conserved regions are amplified with PCR primers and sequenced
Shotgun Metagenomics
This method is non-discriminant in that it will sequence everything in your sample.
Amplicon Sequencing
16S amplicon sequencing is commonly used for identifying and classifying bacteria.
In this 3 Days Masterclass on Metagenomics & Metatranscriptomics we will be focusing on 16s Amplicon Sequencing.
Detailed Agenda of The Masterclass
A detailed overview of how the masterclass will proceed and what all topics will be covered each day so that participants can also be prepared before hand for each day session and get to know how intensive this masterclass will be
Day 1 : August 24, 2023; Thursday
Introduction to Metagenomics & Metatranscriptomics.
Tea/Coffee & Stretching Break
Understanding the Data Types & Going through the process workflow.
Lunch Break
Installing the Required Packages & Setting up the Environment for running the process.
Tea/Coffee & Stretching Break
Retrieving the Data for 16s Amplicon data for running metagenomics & metatranscriptomics analysis.
Q&A and Doubt Solving Session.
Day 2: August 25, 2023; Friday
Sequence quality control and feature table construction & Generating a phylogenetic tree
Tea/Coffee & Stretching Break
Taxonomic classification, Filtering data, Alpha rarefaction plots & diversity analysis
Lunch Break
Longitudinal data analysis, Volatility visualization, Differential abundance testing & Meta-analysis.
Tea/Coffee & Stretching Break
Understanding and interpreting all the results generated during the previous steps, to connect the dots.
Q&A and Doubt Solving Session.
Day 3: August 26, 2023; Saturday
Joining the paired-end data, analyzing the quality of data & removing low quality sequences.
Tea/Coffee & Stretching Break
Removing rRNA reads from data, retrieving the reference sequencing, Mapping of our sequences.
Lunch Break
Subsystem Analysis, RefSeq Analysis & Differential Expression Analysis
Tea/Coffee & Stretching Break
Generating the Subsystem analysis plot & DE Analysis plot and interpreting the results.
Q&A and Doubt Solving Session. Certificate Distribution & Concluding the Masterclass.
TakeAway From The Masterclass
Apart from the topics mentioned above there are a few extra things which you can take away from this bootcamp, which will be adding more value to your work
A Participations certificate is a must after successfully completing the training as a sign of accomplishment.
During the sessions we will be using 2 different case studies, in order to better deal with the topic.
A complete step-wise protocol will also be provided to take help from during & after the masterclass.
During the course of 3 days we will be have live 20+ hourse of training sessions with the participants.
An introductory theory document to help you better understand the subject will also be provided.
Hands-On exercises are a must to better learn any technology and be able to reproduce it later.
Expected Outcomes of the Masterclass
After completing all the tasks of this masterclass all of our participants will be able to:
Recognize and select the right analysis required based on the data type which will be very beneficial for carrying out their own analysis.
Generating the results after completing the whole process and be able to make the sensible interpretation from the results and understand the outcomes.
Get familiar with the process of microbial genome data analysis with different command based tools for different datasets & be able to generate the results.
Be to carryout basic trouble shooting, just like mathematics in bioinformatics every other data-set is different hence require different approch for running and general trobleshooting.
LOCATION
New Delhi, India
DATE & TIME
AUGUST 24-26, 2023 | 10:00 AM - 05:00 PM
VENUE
To Be Announced
Registration Fee
Early Bird Registration Fee – ₹ 3500/- Only
Early-Bird Registration Ends on
August 05, 2023
Terms & Conditions
- All fee paid is not refundable so please read all the terms & conditions before making any payments. If you still have any doubts please contact us and confirm and then only make the payment.
- Participants need to bring their registration tickets along with a valid Institutional ID, then only they will be allowed to attend the session. Please reach out to our team in case of any exceptions.
- Please fill all your details in the form correctly as those details will be used in your certificate as well.
- Participants need to bring their own computer (laptop) system for the program.
- The software tools and other required software tools will be provided from our side for the purpose of this program.
- Participants need to reach the venue and report 30 minutes prior to the start of the sessions.
- Participants need to wear masks all the time inside the premises and abide by the other rules at the premises.
- Particpants need to attend all the sessions in order to be eligible for getting the certificate.
- Welcome email will be sent to all the participants with all the details related to the program. Please check your Inbox/Spam folder for the email.
- All the details of the software installations and how to prepare your system for the Program will be shared with all the participants in the Welcome Email itself.
Contact Us
We understand that you may have some questions before you make the payment for the course. Feel free to get in contact with us through the below given options.
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